Photophosphorylation of base-modified nucleotide analogs by spinach chloroplasts
نویسندگان
چکیده
منابع مشابه
Photophosphorylation by digitonin-fragmented spinach chloroplasts.
Digitonin has been used successfully to disrupt particulate structures such as mitochondria without loss of their capacity for causing oxidative phosphorylation (1, 2). The present report describes a procedure whereby spinach chloroplasts were extensively disrupted by digitonin extraction to give a preparation of small fragments which could cause an appreciable rate of photophosphorylation. In ...
متن کاملInhibition of photophosphorylation in spinach chloroplasts by inorganic sulfate.
Illumination of chloroplasts in the presence of sulfate, ADP, and Mg2+ causes 35 to 50% inhibition of a subsequent phosphorylation reaction; ADP cannot be replaced by other nucleotides. Compounds that normally inhibit photophosphorylation prevent the appearance of the sulfate-induced inhibition. No evidence was found for phosphorylation site specificity of the sulfate inhibition. In chloroplast...
متن کاملRole of AMP in photophosphorylation by spinach chloroplasts.
ATP formation was studied under limited turnover conditions during a postillumination period to ascertain what role, if any, AMP plays in this process. Spinach chloroplast fragments devoid of the conventional adenylate kinase (EC 2.7.4.3) were illuminated in the presence of [32P]orthophosphate and AMP at pH 7.0, and then immediately after the light was turned off, ADP was added. The amount and ...
متن کاملSite-specific Inhibition of Photophosphorylation in Isolated Spinach Chloroplasts by Mercuric Chloride.
Photophosphorylation associated with noncyclic electron transport in isolated spinach (Spinacia oleracea) chloroplasts is inhibited to approximately 50% by low concentrations of HgCl(2) (less than 1 mumole Hg(2+)/mg chlorophyll) when the electron transport pathway includes both sites of energy coupling. Reactions involving only a part of the electron transport system can give a functional isola...
متن کاملInfluence of adenine nucleotides on the inhibition of photophosphorylation in spinach chloroplasts by N-ethylmaleimide.
The incubation of spinach chloroplasts with 1 mM N-ethylmaleimide in light for 60 to 90 s results in a partial, irreversible inhibition of photophosphorylation. The inhibition was not overcome at infinite light intensity or at infinite concentrations of the phosphorylation substrates. Although the inhibition diminished with decreasing concentrations of adenosine diphosphate in the assay of phos...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1979
ISSN: 0014-5793
DOI: 10.1016/0014-5793(79)80739-5